Mafuta oyera a Artemisia capillaris amakandulo ndi sopo kupanga mafuta ophatikizira mafuta ofunikira atsopano kwa zoyatsira bango

Mafuta oyera a Artemisia capillaris a makandulo ndi sopo opangira mafuta ophatikizira mafuta ofunikira atsopano kwa zoyatsira bango

Kufotokozera mwachidule:

Mapangidwe amtundu wa rodent

Nyamazo zinagawidwa mwachisawawa m’magulu asanu a mbewa khumi ndi zisanu gulu lililonse. Gulu loyang'anira ndi mbewa zamagulu amitundu zidagundidwa nazomafuta a sesamekwa masiku 6. Makoswe a gulu loyang'anira bwino adapakidwa mapiritsi a bifendate (BT, 10 mg/kg) kwa masiku 6. Magulu oyeserawo amathandizidwa ndi 100 mg / kg ndi 50 mg / kg AEO osungunuka mu mafuta a sesame kwa masiku 6. Patsiku la 6, gulu lolamulira lidathandizidwa ndi mafuta a sesame, ndipo magulu ena onse adathandizidwa ndi mlingo umodzi wa 0.2% CCl4 mu mafuta a sesame (10 ml / kg) ndi.jakisoni wa intraperitoneal. Kenako mbewa zinasala kudya popanda madzi, ndipo zitsanzo za magazi zinasonkhanitsidwa kuchokera ku ziwiya za retrobulbar; anasonkhanitsa magazi anali centrifuged pa 3000 ×gkwa mphindi 10 kuti alekanitse seramu.Kusamuka kwa khomo lachiberekeroanachitidwa atangochotsa magazi, ndipo zitsanzo za chiwindi zinachotsedwa mwamsanga. Gawo limodzi lachiwindi lidasungidwa nthawi yomweyo pa −20 ° C mpaka kufufuzidwa, ndipo gawo lina linadulidwa ndikuyikidwa mu 10%.formalinyankho; minyewa yotsalayo inasungidwa pa -80 ° C kuti ifufuze za histopathological (Wang et al., 2008,Hsu et al., 2009,Ndi et al., 2015).

Kuyeza kwa magawo a biochemical mu seramu

Kuvulala kwa chiwindi kunayesedwa poyerekezera ndintchito za enzymaticseramu ALT ndi AST pogwiritsa ntchito zida zamalonda zofananira malinga ndi malangizo a zida (Nanjing, Province la Jiangsu, China). Zochita za enzymatic zidawonetsedwa ngati mayunitsi pa lita (U / l).

Kuyeza kwa MDA, SOD, GSH ndi GSH-Pxmu chiwindi homogenates

Chiwindi zimakhala ndi homogenized ndi ozizira zokhudza thupi saline pa chiŵerengero cha 1: 9 (w / v, chiwindi: saline). Ma homogenates anali centrifuged (2500 ×gkwa 10 min) kuti asonkhanitse amatsenga kuti adziwe zomwe zatsatira. Kuwonongeka kwa chiwindi kunayesedwa molingana ndi kuyeza kwa chiwindi kwa milingo ya MDA ndi GSH komanso SOD ndi GSH-P.xntchito. Zonsezi zidatsimikiziridwa potsatira malangizo a zida (Nanjing, Province la Jiangsu, China). Zotsatira za MDA ndi GSH zidawonetsedwa ngati nmol pa mg protein (nmol/mg prot), ndi ntchito za SOD ndi GSH-P.xadawonetsedwa ngati mapuloteni a U pa mg (U / mg prot).

Kusanthula kwa Histopathological

Magawo a chiwindi omwe angopezedwa kumene adakhazikika mu 10% bufferedparaformaldehydephosphate solution. Zitsanzozo zimayikidwa mu parafini, zodulidwa mu magawo 3-5 μm, zodetsedwa ndi.hematoxylinndieosin(H&E) molingana ndi njira yokhazikika, ndipo pomaliza idawunikidwa ndimicroscope yowala(Tian et al., 2012).

Kusanthula kwachiwerengero

Zotsatira zake zidawonetsedwa ngati ± kutembenuka kokhazikika (SD). Zotsatirazo zidawunikidwa pogwiritsa ntchito pulogalamu yowerengera ya SPSS Statistics, mtundu 19.0. Detayo idasinthidwa ndikuwunikiridwa kwa kusiyana (ANOVA,p<0.05) kutsatiridwa ndi mayeso a Dunnett ndi mayeso a T3 a Dunnett kuti adziwe kusiyana kwakukulu pakati pa mikhalidwe yamagulu osiyanasiyana oyesera. Kusiyana kwakukulu kunkaganiziridwa pa mlingo wap<0.05.

Zotsatira ndi zokambirana

Zolemba za AEO

Pakuwunika kwa GC / MS, AEO idapezeka kuti ili ndi zigawo za 25 zochotsedwa ku 10 mpaka 35 min, ndipo zigawo za 21 zomwe zimawerengera 84% yamafuta ofunikira zidadziwika.Table 1). Mafuta ochulukirapo omwe ali nawomonoterpenoids(80.9%), sesquiterpenoids (9.5%), saturated unbranched hydrocarbons (4.86%) ndi miscellaneous acetylene (4.86%). Poyerekeza ndi maphunziro ena (Guo et al., 2004), tapeza ma monoterpenoids (80.90%) mu AEO. Zotsatira zake zidawonetsa kuti gawo lochulukirapo la AEO ndi β-citronellol (16.23%). Zigawo zina zazikulu za AEO zikuphatikizapo 1,8-cineole (13.9%),camphor(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),thymol(3.22%) ndimyrcene(2.02%). Kusiyanasiyana kwa kapangidwe ka mankhwala kumatha kukhala kokhudzana ndi chilengedwe chomwe mbewuyo idakumana nayo, monga madzi amchere, kuwala kwa dzuwa, gawo lachitukuko ndizakudya.

Mtengo wa FOB:US $0.5 - 9,999 / Chigawo

Kuchuluka kwa Min.Order:100 zidutswa / zidutswa

Kupereka Mphamvu:10000 Chidutswa/Zidutswa pamwezi

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Chiwindi matenda, matenda wamba chifukwakachilombo ka hepatitis, uchidakwa, mankhwala owopsa m'chiwindi, zizolowezi zoipa za zakudya ndi kuipitsidwa kwa chilengedwe, ndizodetsa nkhaŵa padziko lonse lapansi (Papay et al., 2009). Komabe, chithandizo chamankhwala cha matendawa nthawi zambiri chimakhala chovuta kupereka ndipo chimakhala chochepa. Chitchainizi Chachikhalidwemankhwala azitsamba, omwe amatsatira malamulo ambiri omwe amagwiritsidwa ntchito pochiza matenda a chiwindi, amagwiritsidwabe ntchito kwambiri ndi achi China (Zhao et al., 2014).Artemisia capillarisThumb.,Asteraceae, malinga ndi Bencao Gangmu, zolemba zodziwika kwambiri za Chinese Traditional Medicine, zakhala zikugwiritsidwa ntchito kwambiri ngati mankhwala ochotsera kutentha, kulimbikitsa.diuresisndi kuchotsa jaundice ndipo amagwiritsidwanso ntchito ngati chokometsera zakumwa, ndiwo zamasamba, ndi makeke chifukwa cha fungo lake lonunkhira.A. capillariswakhala akuonedwa ngati mtundu wa mankhwala achi China ndi chakudya ndi anthu ochuluka. Choncho, pakhala khama lalikulu popanga mankhwala azitsamba othandiza, mongaA. capillaris, zochizira matenda a chiwindi.

M'zaka zaposachedwa, mankhwala azitsamba apeza chidwi komanso kutchuka kwambiri pochiza matenda a chiwindi chifukwa cha chitetezo chawo komanso mphamvu zawo (Ding ndi al., 2012).A. capillariszatsimikiziridwa kuti zili ndi ntchito yabwino ya hepatoprotective kutengera njira zamakono zamankhwala (Han et al., 2006). Ndiwofunikanso mankhwala ku China ndipo ndi otchuka odana ndi kutupa (Cha et al., 2009a),choleretic(Yoon ndi Kim, 2011ndi anti-chotupa (Feng et al., 2013)mankhwala azitsamba.

PhytochemicalKafukufuku wawonetsa kuti mafuta ambiri ofunikira amawonongeka,coumarins,ndiflavonol glycosideskomanso gulu la osadziwikaaglyconeskuchokeraA. capillaris(Komiya et al., 1976,Yamahara et al., 1989). Mafuta ofunikira aA. capillaris(AEO) ndi imodzi mwazinthu zazikulu zomwe zimagwira ntchito pazamankhwala ndipo zimapereka anti-kutupa (Cha et al., 2009andi anti-apoptotic properties (Cha et al., 2009b). Komabe, monga AEO ndi imodzi mwazophatikiza zazikulu zaA. capillaris, kuthekera kwa hepatoprotective ntchito zamagulu akuluakulu kuchokeraA. capillarisziyenera kufufuzidwa.

Mu phunziro ili, chitetezo cha AEO pacarbon tetrachloride(CCl4) -opangidwahepatotoxicityadawunikidwa ndi njira za biochemical, monga hepatickuchepetsa glutathione(GSH),malondialdehyde(MDA) mlingo,superoxide dismutase(SOD), ndiglutathione peroxidase(GSH-Px) ntchito, komanso ntchito zaaspartate aminotransferase(AST) ndialanine aminotransferase(ALT) mu seramu. Kuchuluka kwa kuvulala kwa chiwindi kwa CCl4 kunawunikidwanso kupyolera mu zochitika za histopathological, pamodzi ndi kufufuza kwa phytochemical ndi GC-MS kuti azindikire zigawo za AEO.