OEM Mwambo Phukusi Natural Macrocephalae Rhizoma mafuta

Phukusi la OEM Mwambo Natural Macrocephalae Rhizoma Mafuta Owonetsedwa

Kufotokozera mwachidule:

Monga mankhwala othandiza a chemotherapeutic, 5-fluorouracil (5-FU) amagwiritsidwa ntchito kwambiri pochiza zotupa zowopsa m'mimba, mutu, khosi, chifuwa, ndi ovary. Ndipo 5-FU ndiye mankhwala oyamba a khansa yapakhungu m'chipatala. Kachitidwe ka 5-FU ndikuletsa kusintha kwa uracil nucleic acid kukhala thymine nucleic acid m'ma cell chotupa, kenako kumakhudza kaphatikizidwe ndi kukonzanso kwa DNA ndi RNA kuti akwaniritse cytotoxic zotsatira (Afzal et al., 2009; Ducreux et. al., 2015; Longley et al., 2003). Komabe, 5-FU imapanganso kutsekula m'mimba kwa chemotherapy (CID), imodzi mwazoyipa zomwe zimavutitsa odwala ambiri (Filho et al., 2016). Chiwopsezo cha kutsekula m'mimba mwa odwala omwe amathandizidwa ndi 5-FU chinali mpaka 50% -80%, zomwe zidakhudza kwambiri kupita patsogolo ndi mphamvu ya chemotherapy (Iacovelli et al., 2014; Rosenoff et al., 2006). Chifukwa chake, ndikofunikira kwambiri kupeza chithandizo choyenera cha CID yopangidwa ndi 5-FU.

Pakalipano, njira zosagwirizana ndi mankhwala osokoneza bongo komanso mankhwala osokoneza bongo zatumizidwa ku chithandizo chamankhwala cha CID. Zosagwiritsa ntchito mankhwala osokoneza bongo zimaphatikizapo zakudya zoyenera, komanso kuwonjezera mchere, shuga ndi zakudya zina. Mankhwala monga loperamide ndi octreotide amagwiritsidwa ntchito pochiza matenda otsekula m'mimba a CID (Benson et al., 2004). Kuphatikiza apo, ethnomedicines amatengedwanso kuti azichiza CID ndi mankhwala awo apadera m'maiko osiyanasiyana. Traditional Chinese Medicine (TCM) ndi mtundu umodzi wa ethnomedicine womwe wakhala ukuchitidwa kwa zaka zopitilira 2000 m'maiko aku East Asia kuphatikiza China, Japan ndi Korea (Qi et al., 2010). TCM imanena kuti mankhwala a chemotherapeutic angayambitse kumwa kwa Qi, kusowa kwa ndulu, kusagwirizana m'mimba ndi kunyowa kwa endophytic, zomwe zimapangitsa kuti matumbo asamagwire bwino ntchito. Mu chiphunzitso cha TCM, njira yochiritsira ya CID iyenera kudalira makamaka kuwonjezera Qi ndi kulimbikitsa ndulu (Wang et al., 1994).

Mizu zouma zaAtractylodes macrocephalaKodi. (AM) ndiPanax ginsengCA Mayi. (PG) ndi mankhwala azitsamba omwe amapezeka mu TCM omwe ali ndi zotsatira zofanana zowonjezera Qi komanso kulimbikitsa ndulu (Li et al., 2014). AM ndi PG nthawi zambiri amagwiritsidwa ntchito ngati herb pair (njira yosavuta kwambiri yolumikizana ndi zitsamba zaku China) ndi zotsatira za kuwonjezera Qi ndi kulimbikitsa ndulu pochiza matenda otsekula m'mimba. Mwachitsanzo, AM ndi PG zidalembedwa m'mawu akale odana ndi kutsekula m'mimba monga Shen Ling Bai Zhu San, Si Jun Zi Tang wochokera ku.Taiping Huimin Heji Ju Fang(Song Dynasty, China) ndi Bu Zhong Yi Qi Tang kuchokeraPi Wei Lun(Mzera wa Yuan, China) (mkuyu 1). Maphunziro angapo am'mbuyomu adanenanso kuti mafomu onse atatuwa ali ndi kuthekera kochepetsa CID (Bai et al., 2017; Chen et al., 2019; Gou et al., 2016). Kuphatikiza apo, kafukufuku wathu wam'mbuyomu adawonetsa kuti Shenzhu Capsule yomwe ili ndi AM ndi PG yokha imakhala ndi zotsatirapo zochizira matenda otsekula m'mimba, colitis (xiexie syndrome), ndi matenda ena am'mimba (Feng et al., 2018). Komabe, palibe kafukufuku yemwe wakambirana za momwe AM ndi PG amagwirira ntchito pochiza CID, kaya kuphatikiza kapena kokha.

Tsopano matumbo a microbiota amawerengedwa kuti ndi omwe angathandize kumvetsetsa njira zochiritsira za TCM (Feng et al., 2019). Kafukufuku wamakono akuwonetsa kuti gut microbiota imagwira ntchito yofunika kwambiri pakusunga matumbo a homeostasis. Healthy gut microbiota imathandizira chitetezo cham'mimba mucosal, metabolism, immune homeostasis ndi kuyankha, komanso kuponderezana kwa tizilombo toyambitsa matenda (Thursby ndi Juge, 2017; Pickard et al., 2017). Disordered gut microbiota imasokoneza magwiridwe antchito amthupi ndi chitetezo chamthupi la munthu mwachindunji kapena mwanjira ina, zomwe zimapangitsa kuti pakhale zochitika zam'mbali monga kutsekula m'mimba (Patel et al., 2016; Zhao ndi Shen, 2010). Kafukufuku adawonetsa kuti 5-FU idasintha modabwitsa kapangidwe ka matumbo a microbiota mu mbewa zotsegula m'mimba (Li et al., 2017). Chifukwa chake, zotsatira za AM ndi PM pa kutsekula m'mimba kwa 5-FU zitha kulumikizidwa ndi gut microbiota. Komabe, ngati AM ndi PG okha komanso ophatikizana atha kuteteza kutsekula m'mimba kwa 5-FU posintha matumbo a microbiota sikudziwikabe.

Kuti tifufuze zotsutsana ndi kutsekula m'mimba komanso njira zoyambira za AM ndi PG, tidagwiritsa ntchito 5-FU kutengera chitsanzo cha kutsekula m'mimba mu mbewa. Apa, tidayang'ana pa zotsatira zomwe zingakhalepo za single and integrated administration (AP) yaAtractylodes macrocephalamafuta ofunikira (AMO) ndiPanax ginsengma saponins okwana (PGS), zigawo zogwira ntchito zomwe zimachotsedwa ku AM ndi PG, pa kutsekula m'mimba, matenda a m'mimba ndi tizilombo toyambitsa matenda pambuyo pa 5-FU chemotherapy.

Titumizireni imelo

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Kufunika kwa Ethnopharmacological

Traditional Chinese mankhwala(TCM) imanena kuti kusowa kwa spleen-Qi ndiye vuto lalikulu la kutsekula m'mimba komwe kumachitika chifukwa cha chemotherapy (CID). Herb awiriAtractylodesmacrocephalaKodi. (AM) ndiPanax ginsengCA Mayi. (PG) imakhala ndi zotsatira zabwino zowonjezera Qi ndikulimbitsa ndulu.

Cholinga cha phunziroli

Kufufuza zotsatira zochiritsira ndi njira yaAtractylodes macrocephalamafuta ofunikira (AMO) ndiPanax ginsengzonsesaponins(PGS) yokha komanso yophatikiza (AP) pa 5-fluorouracil (5-FU) chemotherapy idayambitsa kutsekula m'mimba mu mbewa.

Zida ndi njira

Makoswe amaperekedwa ndi AMO, PGS ndi AP motsatana kwa masiku a 11, ndipo intraperitoneally jekeseni ndi 5-FU kwa masiku 6 kuyambira tsiku la 3rd la kuyesa. Panthawi yoyesera, kulemera kwa thupi ndi kutsekula m'mimba mbewa zambiri zinkalembedwa tsiku ndi tsiku. Milozo ya thymus ndi ndulu idawerengedwa pambuyo pa nsembe ya mbewa. Kusintha kwa ma pathological mu minofu ya ileamu ndi colonic kudawunikidwa ndi kutayira kwa hematoxylin-eosin (HE). Ndipo kuchuluka kwa ma cytokines otupa m'matumbo adayesedwa ndi ma enzyme-linked immunosorbent assays (ELISA).16S rDNAAmplicon Sequencing idagwiritsidwa ntchito kusanthula ndikutanthauziramicrobiota m'matumboza zitsanzo za ndowe.

Zotsatira

AP idaletsa kwambiri kuwonda kwa thupi, kutsekula m'mimba, kuchepa kwa ma thymus ndi ndulu, komanso kusintha kwa ma ileamu ndimatumbo oyambitsidwa ndi 5-FU. Palibe AMO kapena PGS yokha yomwe idasintha kwambiri zolakwika zomwe tazitchula pamwambapa. Kupatula apo, AP ikhoza kupondereza kwambiri kuwonjezeka kwa 5-FU-mediated kwa intestinal inflammatory cytokines (TNF-α, IFN-γ, IL-6, IL-1βndi IL-17), pamene AMO kapena PGS inangoletsa ena mwa iwo pambuyo pa 5-FU chemotherapy. Kusanthula kwa Gut microbiota kunawonetsa kuti 5-FU idapangitsa kusintha kwamapangidwe amicrobiota m'matumboadasinthidwa pambuyo pa chithandizo cha AP. Kuphatikiza apo, AP idasinthiratu kuchuluka kwa ma phyla osiyanasiyana ofanana ndi abwinobwino, ndikubwezeretsanso ziwerengero zaFirmicutes/Matenda a Bacteroidetes(F/B). Pa mlingo wa jenasi, chithandizo cha AP chinachepetsa kwambiri tizilombo toyambitsa matenda mongaMatenda a Bacteroides,Ruminococcus,AnaerotruncusndiDesulfovibrio. AP idatsutsananso ndi zovuta za AMO ndi PGS zokha pamtundu winaBlautia,ParabacteroidesndiLactobacillus. Palibe AMO kapena PGS yokha yomwe idaletsa kusintha kwa ma microbial m'matumbo chifukwa cha 5-FU.